Tooth extraction tec的問題，透過圖書和論文來找解法和答案更準確安心。 我們找到下列推薦必買和特價產品懶人包

MicroRNA-1調控LITAF過度表現誘使胃癌細胞的生長與侵犯

為了解決Tooth extraction tec的問題，作者陳彥志 這樣論述：

研究目的： 在許多的發炎性疾患都可以觀察到Lipopolysaccharide-induced tumor necrosis factor alpha factor (LITAF)基因的過度表現。此外，LITAF在過多被視為抑癌基因，在許多癌症組織都可觀察LITAF的低表現量。但在胃癌細胞中關於LITAF的可能作用及其機轉，以及胃幽門螺旋桿菌感染與LITAF的關係仍然不明。在此研究中，我們探討LITAF基因在胃癌細胞中之表現並研究microRNA-LITAF axis在胃癌細胞中之表現，及其臨床意義。研究方法：我們首先藉由real-time PCR 的方法以及使用本院生物資料庫及公開資料庫的

檢體及資訊，探討LITAF 在胃癌細胞中之表現及其臨床意義。其次，我們以proliferation assay, colony formation assay, migration and invasion assay 驗證LITAF對胃癌細胞之生長能力與移動性之影響，並以luciferase reporter, western blot and real-time PCR等方法探討miR-1-3p與LITAF之交互影響及其機轉。結果：相較於正常細胞，胃癌細胞的LITAF之表現顯著升高。而較高的LITAF表現量也被證實與較差的預後，較嚴重的癌症期別與較短的生存期相關。我們也發現miR-1-3p

可與LITAF mRNA的3′-UTR結合並抑制胃癌細胞的增生予移行侵犯能力。結論：在胃癌細胞中，miR-1-3p的低表現量造成LITAF的高表現進而影響胃癌的預後。此發現可作為將來治療的目標進而改善胃癌治療的預後。關鍵字：胃癌，LITAF，microRNA-1

Reproductive Physiology of Taiwanese Pangolin (Manis pentadactyla pentadactyla)

為了解決Tooth extraction tec的問題，作者Bharti Arora 這樣論述：

Taiwanese pangolin (Manis pentadactyla pentadactyla) knowledge of reproductive physiology is imperative to maintaining a healthy ex-situ and in-situ population. Pangolins have been exhibited in zoos since the mid-1800s, but these prolonged years of captivity are facing a dilemma to propagate succes

sful breeding in captivity. According to International Studbook and Formosan pangolin PHVA final report, this species' gestation length is confined in skepticism as well as has a low reproductive rate. Moreover, loss of neonates to stillbirths and incorrect maternal care during the critical period o

f pregnancies in captivity. As females do not exhibit clear signs of estrus or any specific sexual behavior and sometimes the signs of pregnancy have not been recognized to facilitate association between males and females to generate good breeding conditions. Pangolins have captivated the attention

to unravel ecological and behavioral parameters, but reports on reproductive endocrinology are sketchy. I studied various reproductive physiology parameters (mating, pregnancy, gestation, and parturition) using serum and fecal samples procured from longitudinal sampling.Fecal samples obtained enable

d me to devise various metabolic forms of adrenal and gonadal steroids with the establishment of a pivotal study using the technique of Electrospray Mass Spectrometry (ESI-MS) existing in these endangered species of pangolins. The study also helped me unveil that Taiwanese pangolin does not show any

significant differences among the functional categories of sub-adult (SA) and adult (A) in both males and females. However, the males showed significantly high differences in the level of fecal glucocorticoids compared with females.The long-term hormonal examination of serum samples of pangolins fr

om captivity illustrated significant differences in serum estradiol concentration between non-pregnant and pregnant females. However, both groups showed that estradiol's peak is synonymous with the annual mating season tied during the end of the year (November to January). The rising levels of proge

sterone in non-pregnant individuals during January suggest that estradiol and progesterone help in ovulation and estrus behavior in females. In pregnant females, the progesterone concentration sustained from January to September and drastically declined to mark the phase of parturition (October-Dece

mber) in this species. In contrast, the non-pregnant females illustrated baseline concentration of serological progesterone apart from the estrus phase as marked in other closely related carnivores. This long gestation period of ~275 days (January-September) witnessed in pregnant females that it is

highly probable that pangolins exhibit delayed implantation phenomena. The prolactin studies explained that pangolins exhibit a brief period of diapause because of the nursing behavior, which lasts from December to May.Interestingly, males showed a seasonal pattern of testosterone levels. The endocr

inological results suggest that the males engage in the mating behavior when the testosterone levels are the highest (October) in synchrony with females' parturition behavior. In summary, the hormonal evidence found both in male and female Taiwanese pangolin suggests that pangolins are seasonal bree

ders. This study also provided needed information on endocrinology and reproductive physiology for a hitherto unstudied species and provided a basis for a crucial contribution to future reproductive measures in conserving this enigmatic mammal.